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The volumetric finding is a hypothesis worth testing in a powered trial with volume change as a prespecified endpoint, not a demonstration that the drug protects brain tissue
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On day 2, slices were washed 3×5 min in PBS 0.01 M at RT – 4 mL/well and incubated in donkey anti-guinea pig-AF647 (1:500, Jackson ImmunoResearch) diluted in [PBS 0.01 M + 0.3% Triton]
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